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HEPG2 2.15細(xì)胞| HEPG2 2.15細(xì)胞株 現(xiàn)貨附STR鑒定
產(chǎn)品名稱：HEPG2 2.15細(xì)胞
種屬 ：人肝癌細(xì)胞
生長(zhǎng)特性 ：貼壁
形態(tài) ：上皮
培養(yǎng)基 ：DMEM high glucose+15%FBS
生長(zhǎng)條件 ：95%空氣+5%二氧化碳 37攝氏度
凍存條件： 90％FBS＋10％DMSO
凍存條件： 90％FBS＋10％DMSO慧穎生物是一家專門從事細(xì)胞培養(yǎng)相關(guān)試劑公司，擁有完善細(xì)胞庫(kù)管理體系，公司4000多株細(xì)胞逐一做STR鑒定，為每個(gè)科研用戶負(fù)責(zé)，提供無(wú)交叉污染，真實(shí)，來(lái)源可靠的細(xì)胞株！
HEPG2 2.15細(xì)胞| HEPG2 2.15細(xì)胞株 現(xiàn)貨附STR鑒定
常見抗生素的配方：
氨芐青霉素
氨芐青霉素（ampicillin）（100mg/ml）縮寫：Amp
溶解1g氨芐青霉素鈉鹽于足量的水中，zui后定容至10ml。分裝成小份于-20℃貯存。常以25ug/ml～50ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。
羧芐青霉素
羧芐青霉素（carbenicillin）（50mg/ml）縮寫：Cab
溶解0.5g羧芐青霉素二鈉鹽于足量的水中，zui后定容至10ml。分裝成小份于-20℃貯存。常以25ug/ml～50ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。
甲氧西林
甲氧西林（methicillin）（100mg/ml）
溶解1g甲氧西林鈉于足量的水中，zui后定容至10ml。分裝成小份于-20℃貯存。常以37.5ug/ml終濃度與100ug/ml氨芐青霉素一起添加于生長(zhǎng)培養(yǎng)基。
卡那霉素
卡那霉素（kanamycin）（10mg/ml）縮寫：Kan
溶解100mg卡那霉素于足量的水中，zui后定容至10ml。分裝成小份于-20℃貯存。常以10ug/ml～50ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。
氯霉素
氯霉素（chloramphenicol）（25mg/ml）縮寫：Cm
溶解250mg氯霉素足量的無(wú)水乙醇中，zui后定容至10ml。分裝成小份于-20℃貯存。常以12.5ug/ml～25ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。
生物實(shí)驗(yàn)高清視頻教程
鏈霉素
鏈霉素（streptomycin）（50mg/ml）縮寫：Sm
溶解0.5g鏈霉素硫酸鹽于足量的無(wú)水乙醇中，zui后定容至10ml。分裝成小份于-20℃貯存。常以10ug/ml～50ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。
萘啶酮酸
萘啶酮酸（nalidixic acid）（5mg/ml）
溶解50mg萘啶酮酸鈉鹽于足量的水中，zui后定容至10ml。分裝成小份于-20℃貯存。常以15ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。
四環(huán)素
四環(huán)素（tetracyyline）（10mg/ml）縮寫：Tet
溶解100mg四環(huán)素鹽酸鹽于足量的水中，或者將無(wú)堿的四環(huán)素溶于無(wú)水乙醇，定容至10ml。分裝成小份用鋁箔包裹裝液管以免溶液見光，于-20℃貯存。常以10ug/ml～50ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。（注：鎂離子是四環(huán)素的拮抗劑，四環(huán)素抗性菌的篩選應(yīng)使用不含鎂鹽的培養(yǎng)基，如LB培養(yǎng)基。）
紅霉素
紅霉素（erythromycin）（20mg/ml）縮寫：Em
容積為95%乙醇或冰醋酸，用錫箔包裹以免溶液見光，于-20℃貯存，稀釋劑為蒸餾水。
（紅霉素和四環(huán)素等在偏酸環(huán)境下抗菌活性是會(huì)下降的，而在偏堿環(huán)境則會(huì)增加，阿米卡星和頭孢類等在偏堿環(huán)境抗菌活性會(huì)下降，在偏酸環(huán)境是會(huì)增加。）
HEPG2 2.15細(xì)胞| HEPG2 2.15細(xì)胞株 現(xiàn)貨附STR鑒定
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